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These vectors are used for inducible expression of polyhistidine-HaloTag™ fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
HisLink Protein Purification Resin is a macroporous silica resin modified to contain a high level of tetradentate-chelated nickel (>20mmol Ni/ml settled resin) for efficient capture and purification of bacterially expressed His- or HQ-tagged proteins.
The NanoBRET Positive Control Vector encodes a NanoLuc-HaloTag™ fusion protein that tethers together the NanoLuc donor and HaloTag™ acceptor proteins to ensure efficient energy transfer.
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in a purified enzyme preparation.
These vectors are used for inducible expression of polyhistidine-HaloTag™ fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.
The Proteasome-Glo™ Cell-Based Assays are homogeneous, luminescent assays that measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells.
These multiple cloning site vectors are used for inducible expression of HaloTag-polyhistidine fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.
NanoBRET Transcriptional Protein Assays are sensitive, reproducible live-cell assays designed for monitoring or screening the interaction of proteins involved in transcriptional regulation.
These vectors are used for inducible expression of polyhistidine-HaloTag™ fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Use the Flexi Cloning System vectors for C- or N-terminal fusions.